Advanced Quantitative Fluorescence Microscopy to Probe the Molecular Dynamics of Viral Entry, Science Lab
Descrição
Viral entry into the host cell requires the coordination of many cellular and viral proteins in a precise order. Modern microscopy techniques are now allowing researchers to investigate these interactions with higher spatiotemporal resolution than ever before. Here we present two examples from the field of HIV research that make use of an innovative quantitative imaging approach as well as cutting edge fluorescence lifetime-based confocal microscopy methods to gain novel insights into how HIV fuses to cell membranes and enters the cell.
HIV-1 binding and fusion events observed using confocal fluorescence
High-Speed AFM Reveals Molecular Dynamics of Human Influenza A Hemagglutinin and Its Interaction with Exosomes
Purification Analysis, Intracellular Tracking, and Colocalization of Extracellular Vesicles Using Atomic Force and 3D Single-Molecule Localization Microscopy
Nonclinical pharmacokinetics and biodistribution of VSV-GP using methods to decouple input drug disposition and viral replication: Molecular Therapy - Methods & Clinical Development
Microbial pathogenesis revealed by intravital microscopy: pros, cons and cautions - Stolp - 2016 - FEBS Letters - Wiley Online Library
Combining confocal and atomic force microscopy to quantify single-virus binding to mammalian cell surfaces
Quantum Dots: A Promising Fluorescent Label for Probing Virus Trafficking
Microscopy in Virology, Science Lab
Chemosensors, Free Full-Text
Advanced Quantitative Fluorescence Microscopy to Probe the Molecular Dynamics of Viral Entry, Science Lab
Biosensors, Free Full-Text
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